Blar i forfatter "Christensen, Mikkel"
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Arctic marine bacteria for bioplastic production
Christensen, Mikkel (Conference object; Konferansebidrag, 2018)<p>Oil-based plastic production now exceeds 330 Mt annually1 and poses a major environmental problem. Polyhydroxyalkanoates (PHA) is a group of bioplastic polyesters that can replace oil-based plastics in many applications, but economic costs are higher and industrial-scale production still limited. PHA is produced naturally by diverse groups of bacteria and stored intracellularly as hydrophobic ... -
Arctic, marine bacteria and their ability to produce polyhydroxyalkonates (PHAs) using underexploited biomass as nutrient source
Christensen, Mikkel (Conference object; Konferansebidrag, 2017)<p>In this project we are studying marine bacteria and their ability to produce polyhydroxyalkonates (PHAs), a fully biodegradable polyester known as bioplastic. Bioplastics can replace petroleum-based plastics in many applications. PHAs accumulate as intracellular carbon and energy storages when the bacteria grow in an environment with nutrient imbalance. The monomeric building blocks in the PHA ... -
High natural PHA production from acetate in Cobetia sp. MC34 and Cobetia marina DSM 4741T and in silico analyses of the genus specific PhaC2 polymerase variant
Christensen, Mikkel; Jablonski, Piotr; Altermark, Bjørn; Irgum, Knut; Hansen, Hilde (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-20)Background: Several members of the bacterial Halomonadacea family are natural producers of polyhydroxyalkanoates (PHA), which are promising materials for use as biodegradable bioplastics. Type-strain species of Cobetia are designated PHA positive, and recent studies have demonstrated relatively high PHA production for a few strains within this genus. Industrially relevant PHA producers may therefore ... -
High natural PHA production from acetate in Cobetia sp. MC34 and Cobetia marina DSM 4741T and in silico analyses of the genus specific PhaC2 polymerase variant
Christensen, Mikkel; Jablonski, Piotr; Altermark, Bjørn; Irgum, Knut; Hansen, Hilde (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-20)<b>Background</b> <p>Several members of the bacterial <i>Halomonadacea</i> family are natural producers of polyhydroxyalkanoates (PHA), which are promising materials for use as biodegradable bioplastics. Type-strain species of <i>Cobetia</i> are designated PHA positive, and recent studies have demonstrated relatively high PHA production for a few strains within this genus. Industrially relevant ... -
Improved CRISPR/Cas9 gene editing by fluorescence activated cell sorting of green fluorescence protein tagged protoplasts
Petersen, Bent Larsen; Möller, Svenning Rune; Mravec, Jozef; Jørgensen, Bodil; Christensen, Mikkel; Liu, Ying; Wandall, Hans H.; Bennett, Eric Paul; Yang, Zhang (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-06-17)<i>Background</i>: CRISPR/Cas9 is widely used for precise genetic editing in various organisms. CRISPR/Cas9 editing may in many plants be hampered by the presence of complex and high ploidy genomes and inefficient or poorly controlled delivery of the CRISPR/Cas9 components to gamete cells or cells with regenerative potential. Optimized strategies and methods to overcome these challenges are ... -
Towards high-throughput screening (HTS) of polyhydroxyalkanoate (PHA) production via Fourier transform infrared (FTIR) spectroscopy of Halomonas sp. R5-57 and Pseudomonas sp. MR4-99
Christensen, Mikkel; Chiciudean, Iulia; Jablonski, Piotr; Tanase, Ana Maria; Shapaval, Volha; Hansen, Hilde (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-03-08)High-throughput screening (HTS) methods for characterization of microbial production of polyhydroxyalkanoates (PHA) are currently under investigated, despite the advent of such systems in related fields. In this study, phenotypic microarray by Biolog PM1 screening of Halomonas sp. R5-57 and Pseudomonas sp. MR4-99 identified 49 and 54 carbon substrates to be metabolized by these bacteria, respectively. ... -
Towards high-throughput screening (HTS) of polyhydroxyalkanoate (PHA) production via Fourier transform infrared (FTIR) spectroscopy of Halomonas sp. R5-57 and Pseudomonas sp. MR4-99
Christensen, Mikkel; Chiciudean, Iulia; Jablonski, Piotr; Tănase, Ana-Maria; Shapaval, Volha; Hansen, Hilde (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-03-08)